Detection of cholera enterotoxin activity in suckling hamsters.

Does enteropathogenic Escherichia coli produce heat-labile enterotoxin, heat-stable enterotoxins a or b, or cholera toxin A subunits?

Although most enteropathogenic Escherichia coli strains do not produce recognized enterotoxins, we wished to examine whether they produce any factors like heat-stable enterotoxin b or cholera toxin active subunits that might be missed by conventional assay methods. E. coli strains E851 (O142) and E2348 (O127) that had caused diarrhea in volunteers were negative for heat-labile enterotoxin and h...

Pathogenesis of vaccine strains of measles virus in suckling hamsters

Evidence for production of an enterotoxin and cholera toxin cross-reactive factor by Aeromonas hydrophila.

The enterotoxins produced by Aeromonas hydrophila were examined for biological activity by the rabbit ileal loop and suckling mouse assays, as well as by elongation of CHO cells. Antigenic evaluation of the culture filtrates from various isolates of A. hydrophila was performed by enzyme-linked immunosorbent assay with anti-cholera toxin and anti-Aeromonas enterotoxin. Heat stability data demons...

Characterization of uterine growth response to cholera toxin in hamsters and test of heat-labile enterotoxin from Escherichia coli.

Cholera toxin (CT) and the heat-labile enterotoxin from Escherichia coli, when injected intraperitoneally into cycling hamsters but not rats or mice, induced a massive uterine growth similar to that normally induced by the implanting blastocyst during pregnancy. CT and heat-labile enterotoxin are the only known agents that have this action in any species. Uterine weight reached a maximal sixfol...

Value of passive immune hemolysis for detection of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli.

The method of passive immune hemolysis of Evans and Evans (Infect. Immun. 16:604-609, 1977) for detection of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli was modified. A total of 373 strains of E. coli were tested by this method using materials obtained by treating the cells with polymyxin B and rabbit antiserum against cholera enterotoxin, purified by affinity gel colum...